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mmp13 inhibitor  (Cayman Chemical)

 
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    Cayman Chemical mmp13 inhibitor
    Mmp13 Inhibitor, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmp13 inhibitor/product/Cayman Chemical
    Average 90 stars, based on 1 article reviews
    mmp13 inhibitor - by Bioz Stars, 2026-02
    90/100 stars

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    mmp13 inhibitor cl 82198  (MedChemExpress)
    94
    MedChemExpress mmp13 inhibitor cl 82198
    A, Heatmap illustrating <t>MMP13</t> expression based on scRNA-seq data across indicated populations in orthotopic PyMT-BO1 tumors from Osx-Cre;TdT mice. B, qPCR analysis showing Mmp13 transcripts in MMFOsx + and MMFctr. C-E, Representative images of crystal violet staining of PyMT-BO1-GFP + tumor cells after 48 hours in 1% FBS control media, or conditioned media (CM) collected from MMFctr and MMFOsx + (C, Bar=200μM). (D) Quantification of area occupied by tumor cells from (C). (E) Number of viable tumor cells in control media, CM from MMFctr or MMFOsx + determined by MTT colorimetric assay. F-G, (F) Representative images of crystal violet staining of PyMT-BO1-GFP + tumor cells treated for 72 hours with control media or CM from MMFOsx + , in the presence or absence of MMP13i (20 μM, Bar=200μM). (G) Quantification of area occupied by the tumor cells in (F). H-I, Representative z-stack images of PyMT spheroids cultured with MMFOsx + and treated with vehicle or 20 µM MMP13i for 5 days (H). Images were converted to 8-bit black-and-white format, and cluster areas were analyzed using ImageJ with a threshold range of 0–73. (I) Quantification of cluster area was performed based on thresholded images in (H). J, Number of viable tumor cells or MMFOsx + treated with vehicle or 20 µM MMP13i for 48 and 72hrs determined by MTT colorimetric assay. K-M, (K) Schematic of MMP13i (0.5mg/mouse) or vehicle treatment of PyMT-BO1-GFP + tumor cell co-injections with MMFctr or MMFOsx + into the mammary fat pad of WT female mice. (L) Tumor volume measured by caliper with data represented as fold change from day 7 and (M) harvested tumors. Results are shown as mean +/- SD. Unpaired Student T-test was performed to determine significance in B and I. One-way ANOVA followed by Turkeýs multiple-comparison test was used to assess significance in D and E. Two-way ANOVA followed by Turkeýs multiple-comparison test was used to determine significance in G, J, and L. (* P≤0.05, **P≤0.01,***P≤0.001, ****P≤0.0001).
    Mmp13 Inhibitor Cl 82198, supplied by MedChemExpress, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmp13 inhibitor cl 82198/product/MedChemExpress
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    t 26c mmp13 inhibitor selleck chemicals s0341  (Selleck Chemicals)
    94
    Selleck Chemicals t 26c mmp13 inhibitor selleck chemicals s0341
    Figure 6. METs facilitate neutrophil transurothelial migration by releasing <t>MMP13</t> (A and B) Immunofluorescence staining of neutrophils in bladder sections from control and PVM-depleted mice at 6 hpi (A). Neutrophil numbers within the ur- othelium were quantified (B). The epithelial layer is outlined by white dash lines. (C) Flow cytometry analysis of neutrophil numbers in the urine from control and PVM-depleted mice (n = 5 mice per group) at 6 hpi. (D and E) Immunofluorescence staining of neutrophils in bladder sections from WT and Pad4/ mice at 6 hpi (D). Neutrophil numbers within the urothelium were quantified (E). (F) Flow cytometry analysis of neutrophil numbers in the urine from WT and Pad4/ mice (n = 4 mice per group) at 6 hpi.
    T 26c Mmp13 Inhibitor Selleck Chemicals S0341, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mmp13 inhibitor way1700523  (Tocris)
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    Tocris mmp13 inhibitor way1700523
    Figure 6. METs facilitate neutrophil transurothelial migration by releasing <t>MMP13</t> (A and B) Immunofluorescence staining of neutrophils in bladder sections from control and PVM-depleted mice at 6 hpi (A). Neutrophil numbers within the ur- othelium were quantified (B). The epithelial layer is outlined by white dash lines. (C) Flow cytometry analysis of neutrophil numbers in the urine from control and PVM-depleted mice (n = 5 mice per group) at 6 hpi. (D and E) Immunofluorescence staining of neutrophils in bladder sections from WT and Pad4/ mice at 6 hpi (D). Neutrophil numbers within the urothelium were quantified (E). (F) Flow cytometry analysis of neutrophil numbers in the urine from WT and Pad4/ mice (n = 4 mice per group) at 6 hpi.
    Mmp13 Inhibitor Way1700523, supplied by Tocris, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mmp13 specific inhibitor  (Millipore)
    90
    Millipore mmp13 specific inhibitor
    Figure 6. METs facilitate neutrophil transurothelial migration by releasing <t>MMP13</t> (A and B) Immunofluorescence staining of neutrophils in bladder sections from control and PVM-depleted mice at 6 hpi (A). Neutrophil numbers within the ur- othelium were quantified (B). The epithelial layer is outlined by white dash lines. (C) Flow cytometry analysis of neutrophil numbers in the urine from control and PVM-depleted mice (n = 5 mice per group) at 6 hpi. (D and E) Immunofluorescence staining of neutrophils in bladder sections from WT and Pad4/ mice at 6 hpi (D). Neutrophil numbers within the urothelium were quantified (E). (F) Flow cytometry analysis of neutrophil numbers in the urine from WT and Pad4/ mice (n = 4 mice per group) at 6 hpi.
    Mmp13 Specific Inhibitor, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    mmp13 inhibitor  (Cayman Chemical)
    90
    Cayman Chemical mmp13 inhibitor
    Figure 6. METs facilitate neutrophil transurothelial migration by releasing <t>MMP13</t> (A and B) Immunofluorescence staining of neutrophils in bladder sections from control and PVM-depleted mice at 6 hpi (A). Neutrophil numbers within the ur- othelium were quantified (B). The epithelial layer is outlined by white dash lines. (C) Flow cytometry analysis of neutrophil numbers in the urine from control and PVM-depleted mice (n = 5 mice per group) at 6 hpi. (D and E) Immunofluorescence staining of neutrophils in bladder sections from WT and Pad4/ mice at 6 hpi (D). Neutrophil numbers within the urothelium were quantified (E). (F) Flow cytometry analysis of neutrophil numbers in the urine from WT and Pad4/ mice (n = 4 mice per group) at 6 hpi.
    Mmp13 Inhibitor, supplied by Cayman Chemical, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    primary antibodies mmp13  (Proteintech)
    92
    Proteintech primary antibodies mmp13
    Histological and immunofluorescence staining of intra-articular injection of rapamycin (A) . The result of HE staining (Scale bar: the left panel was 200 um, the right panel was 25 um) (B) . The result of Toluidine blue staining (Scale bar: the left panel was 200 um, the right panel was 25 um) (C) . The immunofluorescence results of Col2 (Scale bar: the left panel was 200 um, the right panel was 25 um) (D) . The immunofluorescence results of <t>MMP13</t> (Scale bar: the left panel was 200um, the right panel was 25 um) (E) . HC/CC ratio under HE staining (F) . HC/CC ratio under toluidine blue staining (G) . The statistics of Col2 -positive cells (H) . The statistics of MMP13-positive cells. (I) . OARSI score in each group.
    Primary Antibodies Mmp13, supplied by Proteintech, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/primary antibodies mmp13/product/Proteintech
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    primary antibodies mmp13 - by Bioz Stars, 2026-02
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    mmp13 inhibitor 444283  (Millipore)
    90
    Millipore mmp13 inhibitor 444283
    Histological and immunofluorescence staining of intra-articular injection of rapamycin (A) . The result of HE staining (Scale bar: the left panel was 200 um, the right panel was 25 um) (B) . The result of Toluidine blue staining (Scale bar: the left panel was 200 um, the right panel was 25 um) (C) . The immunofluorescence results of Col2 (Scale bar: the left panel was 200 um, the right panel was 25 um) (D) . The immunofluorescence results of <t>MMP13</t> (Scale bar: the left panel was 200um, the right panel was 25 um) (E) . HC/CC ratio under HE staining (F) . HC/CC ratio under toluidine blue staining (G) . The statistics of Col2 -positive cells (H) . The statistics of MMP13-positive cells. (I) . OARSI score in each group.
    Mmp13 Inhibitor 444283, supplied by Millipore, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/mmp13 inhibitor 444283/product/Millipore
    Average 90 stars, based on 1 article reviews
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    Image Search Results


    A, Heatmap illustrating MMP13 expression based on scRNA-seq data across indicated populations in orthotopic PyMT-BO1 tumors from Osx-Cre;TdT mice. B, qPCR analysis showing Mmp13 transcripts in MMFOsx + and MMFctr. C-E, Representative images of crystal violet staining of PyMT-BO1-GFP + tumor cells after 48 hours in 1% FBS control media, or conditioned media (CM) collected from MMFctr and MMFOsx + (C, Bar=200μM). (D) Quantification of area occupied by tumor cells from (C). (E) Number of viable tumor cells in control media, CM from MMFctr or MMFOsx + determined by MTT colorimetric assay. F-G, (F) Representative images of crystal violet staining of PyMT-BO1-GFP + tumor cells treated for 72 hours with control media or CM from MMFOsx + , in the presence or absence of MMP13i (20 μM, Bar=200μM). (G) Quantification of area occupied by the tumor cells in (F). H-I, Representative z-stack images of PyMT spheroids cultured with MMFOsx + and treated with vehicle or 20 µM MMP13i for 5 days (H). Images were converted to 8-bit black-and-white format, and cluster areas were analyzed using ImageJ with a threshold range of 0–73. (I) Quantification of cluster area was performed based on thresholded images in (H). J, Number of viable tumor cells or MMFOsx + treated with vehicle or 20 µM MMP13i for 48 and 72hrs determined by MTT colorimetric assay. K-M, (K) Schematic of MMP13i (0.5mg/mouse) or vehicle treatment of PyMT-BO1-GFP + tumor cell co-injections with MMFctr or MMFOsx + into the mammary fat pad of WT female mice. (L) Tumor volume measured by caliper with data represented as fold change from day 7 and (M) harvested tumors. Results are shown as mean +/- SD. Unpaired Student T-test was performed to determine significance in B and I. One-way ANOVA followed by Turkeýs multiple-comparison test was used to assess significance in D and E. Two-way ANOVA followed by Turkeýs multiple-comparison test was used to determine significance in G, J, and L. (* P≤0.05, **P≤0.01,***P≤0.001, ****P≤0.0001).

    Journal: bioRxiv

    Article Title: Myofibroblastic CAFs arising from bone-resident osteoblast precursors retain an osteolineage signature and support breast cancer progression via Osterix-mediated signaling

    doi: 10.1101/2025.09.18.677154

    Figure Lengend Snippet: A, Heatmap illustrating MMP13 expression based on scRNA-seq data across indicated populations in orthotopic PyMT-BO1 tumors from Osx-Cre;TdT mice. B, qPCR analysis showing Mmp13 transcripts in MMFOsx + and MMFctr. C-E, Representative images of crystal violet staining of PyMT-BO1-GFP + tumor cells after 48 hours in 1% FBS control media, or conditioned media (CM) collected from MMFctr and MMFOsx + (C, Bar=200μM). (D) Quantification of area occupied by tumor cells from (C). (E) Number of viable tumor cells in control media, CM from MMFctr or MMFOsx + determined by MTT colorimetric assay. F-G, (F) Representative images of crystal violet staining of PyMT-BO1-GFP + tumor cells treated for 72 hours with control media or CM from MMFOsx + , in the presence or absence of MMP13i (20 μM, Bar=200μM). (G) Quantification of area occupied by the tumor cells in (F). H-I, Representative z-stack images of PyMT spheroids cultured with MMFOsx + and treated with vehicle or 20 µM MMP13i for 5 days (H). Images were converted to 8-bit black-and-white format, and cluster areas were analyzed using ImageJ with a threshold range of 0–73. (I) Quantification of cluster area was performed based on thresholded images in (H). J, Number of viable tumor cells or MMFOsx + treated with vehicle or 20 µM MMP13i for 48 and 72hrs determined by MTT colorimetric assay. K-M, (K) Schematic of MMP13i (0.5mg/mouse) or vehicle treatment of PyMT-BO1-GFP + tumor cell co-injections with MMFctr or MMFOsx + into the mammary fat pad of WT female mice. (L) Tumor volume measured by caliper with data represented as fold change from day 7 and (M) harvested tumors. Results are shown as mean +/- SD. Unpaired Student T-test was performed to determine significance in B and I. One-way ANOVA followed by Turkeýs multiple-comparison test was used to assess significance in D and E. Two-way ANOVA followed by Turkeýs multiple-comparison test was used to determine significance in G, J, and L. (* P≤0.05, **P≤0.01,***P≤0.001, ****P≤0.0001).

    Article Snippet: When indicated, the MMP13 inhibitor CL-82198 (MedChem Express) dissolved in 10% DMSO and diluted in 40%PEG300 and 50% PBS was injected intravenously (0.5 mg/mouse) to tumor-bearing mice for 10 consecutive days.

    Techniques: Expressing, Staining, Control, Colorimetric Assay, Cell Culture, Comparison

    A, Representative mIHC images of triple-negative (n=10), and ER + (n=8) human breast cancer tissues stained for Osx (red), Hematoxylin (gray), αSMA (blue), PDGFRα (green) and panCK (cyan). The yellow inset highlights the stromal area, and the orange inset highlights the tumor area. B, Representative mIHC images of TNBC patient biopsies at diagnosis before receiving neoadjuvant standard of care. Based on annotated therapeutic response, patients were classified as responders (R=8) or no responders (NR=7). Tissues were stained for Osx (red), Hematoxylin (gray), αSMA (blue), PDGFRα (green), and panCK (cyan). C, Quantification of myCAFs (PDGFRα + /αSMA + cells) within the stroma (PanCK neg cells) in responders vs. non-responders. D, Quantification of Osx + myCAFs (Osx + PDGFRα + αSMA + cells) within the stroma (PanCK neg cells) in responders vs. non-responders. E, Representative mIHC performed as described in (B) on the same tissue samples, with the addition of MMP13 antibody. F, Quantification of MMP13 + cells within the stroma (PanCK neg cells) in responders vs. non-responders. G, Quantification of MMP13 + Osx + myCAFs (PDGFRα + /αSMA + cells) out of total myCAFs in responders vs. non-responders. H, Kaplan-Meier plots of overall survival for breast cancer patients segmented by high (red line) or low (blue line) abundance of orthologous Osx + myCAF using the TCGA-BRAC database. Results are shown as mean +/- SD. Unpaired Student T-test was performed to determine the significance in C,D, F, G, and H. (* P≤0.05, **P≤0.01).

    Journal: bioRxiv

    Article Title: Myofibroblastic CAFs arising from bone-resident osteoblast precursors retain an osteolineage signature and support breast cancer progression via Osterix-mediated signaling

    doi: 10.1101/2025.09.18.677154

    Figure Lengend Snippet: A, Representative mIHC images of triple-negative (n=10), and ER + (n=8) human breast cancer tissues stained for Osx (red), Hematoxylin (gray), αSMA (blue), PDGFRα (green) and panCK (cyan). The yellow inset highlights the stromal area, and the orange inset highlights the tumor area. B, Representative mIHC images of TNBC patient biopsies at diagnosis before receiving neoadjuvant standard of care. Based on annotated therapeutic response, patients were classified as responders (R=8) or no responders (NR=7). Tissues were stained for Osx (red), Hematoxylin (gray), αSMA (blue), PDGFRα (green), and panCK (cyan). C, Quantification of myCAFs (PDGFRα + /αSMA + cells) within the stroma (PanCK neg cells) in responders vs. non-responders. D, Quantification of Osx + myCAFs (Osx + PDGFRα + αSMA + cells) within the stroma (PanCK neg cells) in responders vs. non-responders. E, Representative mIHC performed as described in (B) on the same tissue samples, with the addition of MMP13 antibody. F, Quantification of MMP13 + cells within the stroma (PanCK neg cells) in responders vs. non-responders. G, Quantification of MMP13 + Osx + myCAFs (PDGFRα + /αSMA + cells) out of total myCAFs in responders vs. non-responders. H, Kaplan-Meier plots of overall survival for breast cancer patients segmented by high (red line) or low (blue line) abundance of orthologous Osx + myCAF using the TCGA-BRAC database. Results are shown as mean +/- SD. Unpaired Student T-test was performed to determine the significance in C,D, F, G, and H. (* P≤0.05, **P≤0.01).

    Article Snippet: When indicated, the MMP13 inhibitor CL-82198 (MedChem Express) dissolved in 10% DMSO and diluted in 40%PEG300 and 50% PBS was injected intravenously (0.5 mg/mouse) to tumor-bearing mice for 10 consecutive days.

    Techniques: Staining, Biomarker Discovery, Clinical Proteomics

    Figure 6. METs facilitate neutrophil transurothelial migration by releasing MMP13 (A and B) Immunofluorescence staining of neutrophils in bladder sections from control and PVM-depleted mice at 6 hpi (A). Neutrophil numbers within the ur- othelium were quantified (B). The epithelial layer is outlined by white dash lines. (C) Flow cytometry analysis of neutrophil numbers in the urine from control and PVM-depleted mice (n = 5 mice per group) at 6 hpi. (D and E) Immunofluorescence staining of neutrophils in bladder sections from WT and Pad4/ mice at 6 hpi (D). Neutrophil numbers within the urothelium were quantified (E). (F) Flow cytometry analysis of neutrophil numbers in the urine from WT and Pad4/ mice (n = 4 mice per group) at 6 hpi.

    Journal: Immunity

    Article Title: A bladder-blood immune barrier constituted by suburothelial perivascular macrophages restrains uropathogen dissemination.

    doi: 10.1016/j.immuni.2025.02.002

    Figure Lengend Snippet: Figure 6. METs facilitate neutrophil transurothelial migration by releasing MMP13 (A and B) Immunofluorescence staining of neutrophils in bladder sections from control and PVM-depleted mice at 6 hpi (A). Neutrophil numbers within the ur- othelium were quantified (B). The epithelial layer is outlined by white dash lines. (C) Flow cytometry analysis of neutrophil numbers in the urine from control and PVM-depleted mice (n = 5 mice per group) at 6 hpi. (D and E) Immunofluorescence staining of neutrophils in bladder sections from WT and Pad4/ mice at 6 hpi (D). Neutrophil numbers within the urothelium were quantified (E). (F) Flow cytometry analysis of neutrophil numbers in the urine from WT and Pad4/ mice (n = 4 mice per group) at 6 hpi.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Bacterial and virus strains CFT073 Isolated from patient N/A UTI89 Isolated from patient N/A CFT073-vsfGFP (Kanamycin+) This paper N/A CFT073 (Gentamycin+) This paper N/A Chemicals, peptides, and recombinant proteins Liberase Roche Cat#5401119001 DNase I Roche Cat#11284932001 Zombie Aqua Fixable Viability Kit BioLegend Cat#423102 Precision Count Beads BioLegend Cat#424902 Tamoxifen Sigma-Aldrich T5648; CAS: 10540-29-1 Diphtheria toxin (DT) Sigma-Aldrich D0564 TRITC-dextran Sigma-Aldrich T1287 Cl-amidine: Pad inhibitor Selleck Chemicals S8141;CAS: 1043444-18-3 T-26c: MMP13 inhibitor Selleck Chemicals S0341;CAS: 869296-13-9 SC-236: COX-2 inhibitor Selleck Chemicals S0762; CAS: 170569-86-5 Setanaxib (GKT137831): ROS inhibitor Selleck Chemicals S S7171; CAS: 1218942-37-0 AF594-WGA Invitrogen W11262 SYTOX Green Nucleic Acid Stain Invitrogen S7020 SYTOX Orange Nucleic Acid Stain Invitrogen S11368 AF405-Phalloidin Invitrogen A30104 DAPI Sigma-Aldrich D9542; CAS:28718-90-3 Hoechst 33342 Biosharp BL803A Ampicillin sodium Sangon Biotech Cat#A610028; CAS: 69-52-3 Vancomycin hydrochloride Sangon Biotech Cat#A600983; CAS: 1404-93-9 Neomycin trisulfate salt hydrate Sangon Biotech Cat#A610366; CAS: 1405-10-3 Metronidazole Sangon Biotech Cat#A600633; CAS: 443-48-1 Kanamycin sulfate Sangon Biotech Cat#A506636; CAS: 25389-94-0 Gentamycin sulfate Sangon Biotech Cat#A506614; CAS: 1405-41-0 PMA Sigma-Aldrich P1585; CAS: 16561-29-8 LPS Sigma-Aldrich L2630 Critical commercial assays Total RNA Extraction Reagent kit Vazyme R401-01 RNeasy Plus Micro Kit Qiagen 74034 HiScript III RT SuperMix Vazyme R323 ChamQ Universal SYBR qPCR Master Mix Vazyme Q711-02 Reactive Oxygen Species Fluorometric Assay Kit Elabscience E-BC-F005 Deposited data ScRNAseq: CD45+ cells from naive mouse bladder This paper GEO: GSE278298 Experimental models: Cell lines Human: HUVEC ATCC CRL-1730 Human: THP-1-ZsGreen This paper N/A Experimental models: Organisms/strains Mouse:C57BL/6 GemPharmatech Co., Ltd N/A Mouse: BALB/c GemPharmatech Co., Ltd N/A Mouse: Cx3cr1GFPCcr2RFP-KI/KO: B6.129(Cg)-Cx3cr1tm1Litt Ccr2tm2.1Ifc/JernJ The Jackson Laboratory JAX: 032127 Mouse: Cx3cr1GFP-KI/KO: B6.129P2(Cg)-Cx3cr1tm1Litt/J The Jackson Laboratory JAX: 005582 (Continued on next page) Immunity 58, 1–17.e1–e6, March 11, 2025 e2

    Techniques: Migration, Staining, Control, Flow Cytometry

    Histological and immunofluorescence staining of intra-articular injection of rapamycin (A) . The result of HE staining (Scale bar: the left panel was 200 um, the right panel was 25 um) (B) . The result of Toluidine blue staining (Scale bar: the left panel was 200 um, the right panel was 25 um) (C) . The immunofluorescence results of Col2 (Scale bar: the left panel was 200 um, the right panel was 25 um) (D) . The immunofluorescence results of MMP13 (Scale bar: the left panel was 200um, the right panel was 25 um) (E) . HC/CC ratio under HE staining (F) . HC/CC ratio under toluidine blue staining (G) . The statistics of Col2 -positive cells (H) . The statistics of MMP13-positive cells. (I) . OARSI score in each group.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Enhancing autophagy and energy metabolism in the meniscus can delay the occurrence of PTOA in ACLT rat

    doi: 10.3389/fcell.2022.971736

    Figure Lengend Snippet: Histological and immunofluorescence staining of intra-articular injection of rapamycin (A) . The result of HE staining (Scale bar: the left panel was 200 um, the right panel was 25 um) (B) . The result of Toluidine blue staining (Scale bar: the left panel was 200 um, the right panel was 25 um) (C) . The immunofluorescence results of Col2 (Scale bar: the left panel was 200 um, the right panel was 25 um) (D) . The immunofluorescence results of MMP13 (Scale bar: the left panel was 200um, the right panel was 25 um) (E) . HC/CC ratio under HE staining (F) . HC/CC ratio under toluidine blue staining (G) . The statistics of Col2 -positive cells (H) . The statistics of MMP13-positive cells. (I) . OARSI score in each group.

    Article Snippet: HE staining solution, Toluidine blue staining powder and safranin O/fast green staining powder was buy from Sigma (United States), SDS-PAGE gel rapid preparation kit was attained from Beyotime Biotechnology (Shanghai)Co., Ltd. rapamycin was acquired from Shanghai yuanye Bio-Technology Co., Ltd., IL-1β was purchased from R&D Systems (Bio-Techne), 3-methyladenine (3-MA, autophagy inhibitor) was produced from TargetMol (United States), primary antibodies MMP13 (18165-1-AP), Adamts5 (Ag26730), P-AKT (Phospho-AKT1 (Ser473)), Beclin 1 (11306-1-AP), LC-3B (18725-1-AP), ULK1 (20986-1-AP), ATG12 (11264-1-AP), COL1 (66761-1-lg) achieved from Proteintech Group, COL2 (GB11021) were procured from Servicebio (China), P-S6K (9208S)/S6K (9202S) were come from Cell Signaling Technology (CST), P62 (ab109012) was purchased from Abcam (Shanghai).

    Techniques: Immunofluorescence, Staining, Injection

    The protective effect of rapamycin on the meniscus extracellular matrix. (A) The expression of MMP13, Col1, and Col2 in Western Blot (B) . The gray value statistics of MMP13 (C) . The gray value statistics of Col1 (D) . The gray value statistics of Col2 (E) . The result of safranin O/fast green staining (Scale bar: the left panel was 200 um, the right panel was 25 um) (F) . The immunofluorescence staining results of MMP13 (Scale bar: the left panel was 200 um, the right panel was 25 um) (G) . The statistics of MMP13-positive cells.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Enhancing autophagy and energy metabolism in the meniscus can delay the occurrence of PTOA in ACLT rat

    doi: 10.3389/fcell.2022.971736

    Figure Lengend Snippet: The protective effect of rapamycin on the meniscus extracellular matrix. (A) The expression of MMP13, Col1, and Col2 in Western Blot (B) . The gray value statistics of MMP13 (C) . The gray value statistics of Col1 (D) . The gray value statistics of Col2 (E) . The result of safranin O/fast green staining (Scale bar: the left panel was 200 um, the right panel was 25 um) (F) . The immunofluorescence staining results of MMP13 (Scale bar: the left panel was 200 um, the right panel was 25 um) (G) . The statistics of MMP13-positive cells.

    Article Snippet: HE staining solution, Toluidine blue staining powder and safranin O/fast green staining powder was buy from Sigma (United States), SDS-PAGE gel rapid preparation kit was attained from Beyotime Biotechnology (Shanghai)Co., Ltd. rapamycin was acquired from Shanghai yuanye Bio-Technology Co., Ltd., IL-1β was purchased from R&D Systems (Bio-Techne), 3-methyladenine (3-MA, autophagy inhibitor) was produced from TargetMol (United States), primary antibodies MMP13 (18165-1-AP), Adamts5 (Ag26730), P-AKT (Phospho-AKT1 (Ser473)), Beclin 1 (11306-1-AP), LC-3B (18725-1-AP), ULK1 (20986-1-AP), ATG12 (11264-1-AP), COL1 (66761-1-lg) achieved from Proteintech Group, COL2 (GB11021) were procured from Servicebio (China), P-S6K (9208S)/S6K (9202S) were come from Cell Signaling Technology (CST), P62 (ab109012) was purchased from Abcam (Shanghai).

    Techniques: Expressing, Western Blot, Staining, Immunofluorescence

    Influence of the meniscus on articular cartilage during the development of PTOA (A) . The immunofluorescence staining results of MMP13 in 1 week, 2 and 3 weeks after ACLT surgery (Scale bar: the left panel was 100 um, the middle and the right panel was 25 um). (B) The statistics of MMP13-positive cells in tibia (C) . The statistics of MMP13-positive cells in meniscus (D) . The expression of Adamts5 and MMP13 in Western Blot (E) . The gray value statistics of Adamts5 (F) The gray value statistics of MMP13.

    Journal: Frontiers in Cell and Developmental Biology

    Article Title: Enhancing autophagy and energy metabolism in the meniscus can delay the occurrence of PTOA in ACLT rat

    doi: 10.3389/fcell.2022.971736

    Figure Lengend Snippet: Influence of the meniscus on articular cartilage during the development of PTOA (A) . The immunofluorescence staining results of MMP13 in 1 week, 2 and 3 weeks after ACLT surgery (Scale bar: the left panel was 100 um, the middle and the right panel was 25 um). (B) The statistics of MMP13-positive cells in tibia (C) . The statistics of MMP13-positive cells in meniscus (D) . The expression of Adamts5 and MMP13 in Western Blot (E) . The gray value statistics of Adamts5 (F) The gray value statistics of MMP13.

    Article Snippet: HE staining solution, Toluidine blue staining powder and safranin O/fast green staining powder was buy from Sigma (United States), SDS-PAGE gel rapid preparation kit was attained from Beyotime Biotechnology (Shanghai)Co., Ltd. rapamycin was acquired from Shanghai yuanye Bio-Technology Co., Ltd., IL-1β was purchased from R&D Systems (Bio-Techne), 3-methyladenine (3-MA, autophagy inhibitor) was produced from TargetMol (United States), primary antibodies MMP13 (18165-1-AP), Adamts5 (Ag26730), P-AKT (Phospho-AKT1 (Ser473)), Beclin 1 (11306-1-AP), LC-3B (18725-1-AP), ULK1 (20986-1-AP), ATG12 (11264-1-AP), COL1 (66761-1-lg) achieved from Proteintech Group, COL2 (GB11021) were procured from Servicebio (China), P-S6K (9208S)/S6K (9202S) were come from Cell Signaling Technology (CST), P62 (ab109012) was purchased from Abcam (Shanghai).

    Techniques: Immunofluorescence, Staining, Expressing, Western Blot